KMID : 0921620200500010035
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Journal of Bacteriology and Virology 2020 Volume.50 No. 1 p.35 ~ p.43
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Stable Expression of Bovine Integrin Beta-6 Increases Susceptibility of Goat Kidney Cell Line to Foot-and-mouth Disease Virus
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Kim Su-Mi
Kim Se-Kyung Lee Kwang-Nyeong Park Jong-Hyeon Kim Byoung-Han
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Abstract
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The integrins ¥áv¥â1, ¥áv¥â3, ¥áv¥â6, and ¥áv¥â8 are known to be the natural receptors of foot-and-mouth disease virus (FMDV). Among them, integrin ¥áv¥â6 is considered a major receptor for FMDV. We performed protein expression of full-length bovine integrins ¥áv, ¥â3, and ¥â6 and confirmed the high efficiency of bovine ¥áv¥â6 as the FMDV receptor in FMDV non-permissive SW 480 cells. Next, we established the black goat kidney (BGK) cell line, stably expressing bovine integrin ¥â6 (BGK-¥â6-4). We observed that BGK-¥â6-4 cells had significantly enhanced sensitivity to FMDV compared with that of BGK cells (P<0.05). In addition, BGK-¥â6-4 cells had equal or higher sensitivity to several serotypes of FMDV compared with that of other FMDV permissive cell lines, such as BHK-21 and IBRS-2. In conclusion, we established a promising novel goat cell line, BGK-¥â6-4, which can be used to isolate or culture FMDV. Furthermore, the BGK-¥â6-4 cell line may serve as a promising tool for studying integrin ¥áv¥â6 receptor functions.
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KEYWORD
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Foot-and-mouth disease virus, goat cell line, integrin ¥áv¥â6
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